New Run | Existing Results | My Primers Paste Sequence   Or Upload File Primer Tm Min: Opt: Max: Primer Size Min: Opt: Max: Product Size Min: Opt: Max: Product CPGs    Primer Poly X Primer non-CPG 'C's    Primer Poly T Maximize Coverage Target Excluded Regions Transcription Region Select Strand forward reverse both Number of output primers Mass Window Low: High: Analyze CpGs in C Reaction Analyze CpGs in T Reaction Notes